Bioremediation of AOX from pulp and paper industry wastewater and AOX contaminated soils
DOI:
https://doi.org/10.15626/Eco-Tech.2005.049Nyckelord:
AOX degradation; Pulp and paper; 2,4 DCP degradation; BioremediationAbstract
Bleaching of paper pulp with chlorine/ chlorine derivatives leads to formation of several
organochlorine compounds. These compounds are collectively termed as "Adsorbable Organic
Halides" (AOX). Toxic effects of AOX are well documented. Analysis of various wastewaters,
soils irrigated with wastewater and sludge show significant levels of AOX. This is a potential
threat to the environment. Physical and chemical methods of AOX removal are not economical.
Hence, bioremediation technology needs to be developed for effective, ecofriendly and
economical management of AOX containing waste. With this aim, anaerobic biofilm reactors
(7.2L) were developed in our laboratory. Anaerobic cultures degrading chlorophenols were
enriched and a consortium of such enrichments was used as the inoculum for the reactors. The
reactors were fed with nutrient medium and run at 20 days HRT and at 30 °c. Degradation of 2,4
dichlorophenol (2,4 DCP) was studied at 100 ppm, 300 ppm and 600 ppm and 96.7, 74.5 and
56.8 % dechlorination was observed, respectively. Anaerobic degradation at varying
concentration of AOX from bleach composite wastewater (BCWW) was studied. Reactors fed
with 12.5, 25, 50 and 75 % BCWW v/v showed 62.9, 56.5, 55.9 and 27.1% AOX degradation,
respectively. The results suggest feasibility of treating the BCWW stream separately in anaerobic
reactor and then mixing the effluent with composite wastewater. The treatment will ensure
prevention of AOX pollution. Fish toxicity bioassays are being carried out to study safe disposal
of treated wastewater in natural water bodies. Aerobic cultures degrading different chlorophenols
were isolated. Using a consortium of these cultures bioremediation studies on AOX contaminated
soils have been initiated.
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Referenser
Freire, C. S. R., Silvestre, A. J. P., Neto, C. P., 2003. Carbohydrate derived chlorinated compounds in ECF bleaching of hardwood pulp: formation, degradation and contribution to AOX in a bleached Kraft pulp mill. Environmental Science and Technology 37, 811 -814. https://doi.org/10.1021/es0200847
Suntio, L. R., Shiu, W. Y., MacKay, D. A., 1988. A review of the nature and properties of the chemicals present in pulp and paper mill effluents. Chemosphere 17 (7), 1249-1290. https://doi.org/10.1016/0045-6535(88)90080-X
Bajpai, P., 2001. Microbial degradation of pollutants in pulp mill effluents. Advances in Applied Microbiology 48, 79-134. https://doi.org/10.1016/S0065-2164(01)48001-4
Fantroussi, El. S., Naveau, H., Agathos, S. N., 1998. Anaerobic dechlorinating bacteria. Biotechnology progress 14, 167-188.
Mohn, W. W., Tiedje, J. M., 1992. Microbial reductive dehalogenation. Microbiology Reviews 56, 482-484.
Dutta, S. K., Boissya, C. L., 1996. Effect of paper mill effluent on germination of rice seed (Oryza saliva L. Var MASURJ) and growth behavior of its seedlings. Journal of Industrial Pollution Control 12 (2), 123- 128.
Palm, H, Lammi, R., 1995. Fate of pulp mill organochlorines in the Gulf of Bothnia sediments. Environmental Science and Technology 29, 1722-1727. https://doi.org/10.1021/es00007a005
Malla, L. , Mohanty, B. K., 2005. Effect of paper mill effluent on germination of green gram (Phaseolus aureus Roxb.) and growth behavior of its seedlings. Journal of Environmental Biology 26(2 suppl), 379-382.
Welker, A., Schmitt, T. G., 1997. A basic investigation in origins of AOX- substances in paper sludges. Water Research 31 (4), 805-815. https://doi.org/10.1016/S0043-1354(96)00284-9
Touzel, J.rP., Albagnac, G., 1983. Isolation and characterization of Methanococcus mazei strain MC3. FEMS Microbiology Letters 16, 241-245. https://doi.org/10.1111/j.1574-6968.1983.tb00295.x
Freedman, D. L., Gossett, J. M., 1989. Biological reductive dechlorination oftetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions. Applied and Environmental Microbiology 55 (9), 2144-2151.
Ranade, D, R,, Gadre, R, V,, 1988, Principles of anaerobic techniques and culture methods. In: Ranade and Gadre (ed.), Microbiological Aspects of Anaerobic Digestion: Laboratory Manual. Published by MACS Research Institute, Pune, India, 15-30.
Becker, J. G., 1999. Reductive dehalogenation and conversion of 2-chlorophenol to 3- chlorobenzoate in methanogenic sediment community. Applied and Environmental Microbiology 65 (11), 5169-5172.
Greenberg, A. E., Clesceri, L, S,, Eaton, A. D., 1992, Standard methods for examination of water and wastewater. ( 18th Ed.). APHA, A WWA and WPCF, Washington DC.
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