Effectiveness of bioremediation process in hydrocarbon - contaminated soils
DOI:
https://doi.org/10.15626/Eco-Tech.2003.011Nyckelord:
Soil bioremediation, bacterial isolates, fuel oil hydrocarbons.Abstract
The present study was undertaken to evaluate the efficacy of introduced indigenous
bacterial isolates for ex situ bioremediation of fuel oil contaminated soil. For this purpose
three hydrocarbon-degrading indigenous bacterial isolates were screened from petroleum
oil contaminated soil and repeatedly used for inoculation of fuel oil contaminated soil.
The total petroleum hydrocarbons (TPH) content was determined by gravimetric method,
Hydrocarbon fractions (alkanes, aromatics, asphaltenes and resins) present in TPH were
obtained by silica gel column chromatography. The study showed that some introduced
bacterial isolates effectively adapted to the contaminated soil. The bioaugmentation effect
was calculated to raise the numbers of bacteria by approximately one order of magnitude
from the indigenous population at the site. Ex situ study showed that the introduced
bacterial consortium effectively adapted to the local environment of the soil at the
bioremediation site.
Our results indicated that disappearance of TPH from inoculated soil samples depended
on the general soil impurity, term of bacterial treatment, level of TPH contamination and
individual microorganism efficacy. With application of bacterial consortium and
fertilizers, the TPH level was reduced to 60 - 66% after three months.
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Referenser
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